Bioremediation of acid mine drainage using sulfate-reducing wetland bioreactor: Filling substrates influence, sulfide oxidation and microbial community.

Two sulfate-reducing wetland bioreactors (SRB-1 filled with lignocellulosic wastes and SRB-2 with river sand) were applied for synthetic acid mine drainage treatment with bio-waste fermentation liquid as electron donor, and the influence of filling substrates on sulfate reduction, sulfur transformation and microbial community was studied. The presence of lignocellulosic wastes (mixture of cow manure, bark, sawdust, peanut shell and straw) in SRB-1 promoted sulfate reduction efficiency (68.9%), sulfate reduction rate (42.1 ± 11 mg S/(L·d)), dissolved sulfide production rate (27.4 ± 7 mg S/(L·d)), and particularly caused high conversion ratio of sulfate reduction into dissolved sulfide (66.4%). In comparison, the relatively low sulfate reduction efficiency (42.9%), sulfate reduction rate (27.0 ± 10 mg S/(L·d)), dissolved sulfide production rate (5.6 ± 3 mg S/(L·d)) and low dissolved sulfide conversion efficiency (21.2%) occurred in SRB-2. Mixed organic substrates including easily assimilated electron donors (in manure) and lignocellulosic matter were effective to promote quick start and long-term microbial sulfate reduction. More than 98% of produced dissolved sulfide was oxidized dominantly by photoautotrophic green sulfur bacteria (genera Chlorobium and Chlorobaculum), of which 64.6% and 54.5% was converted into elemental sulfur for SRB-1 and SRB-2. The oxidation of sulfide into elemental sulfur for potential recovery rather than sulfate is preferred. Diverse sulfate reducing bacteria and sulfide oxidizing bacteria co-existed in the treatment system, which led to a sustainable sulfur transformation. High metal removal efficiency for Fe (99.6%, 92.5%), Cd (99.9%, 99.9%), Zn (99.4%, 98.5%), Cu (94.5%, 94.6%) except for Mn (9.3%, 3.6%) was achieved, and effluent pH increased to 6.5-7.7 and 6.7-7.7 for SRB-1 and SRB-2, respectively. Microbial community was regulated by filling substrates. Synergism between lignocellulosic decomposing bacteria and sulfate reducing bacteria played a vital role in lignocellulosic bioreactor treating AMD, in addition to fermentation liquid serving as effective electron donor.

Effective co-treatment of synthetic acid mine drainage and domestic sewage using multi-unit passive treatment system supplemented with silage fermentation broth as carbon source.

A multi-unit passive treatment system was constructed for co-treatment of synthetic acid mine drainage (AMD) and domestic sewage supplemented with silage fermentation broth as carbon source. AMD and domestic sewage mixing pretreatment (unit 1) improved influent quality with pH increase, metals removal and nutrients supplement. The generated metal-rich sludge in unit 1 retained the metals (69.95% of Fe, 97.36% of Cu, 96.53% of Cd, 72.52% of Zn, and 8.59% of Mn) of influent prior to entering subsequent bioreactors. Silage fermentation broth performed well to promote bacterial sulfate reduction in sulfate reducing bioreactor system (unit 2). Residual metals (Mn) and organic/nutrient pollutants were further polished in surface-flow aerobic wetland (unit 3), where relatively high pH (7.4-8.6), aerobic condition, potential Mn-oxidizing bacteria, limestone layer and low concentrations of Fe(II) (0.04-3.5 mg/L) favored the efficient removal of Mn. After 210-day continuous flow-through experiment, this passive treatment system demonstrated the efficient performance, increasing pH from 2.5 to 8.0 with removal of metals (99%), sulfate and organic/nutrient pollutants. Diverse sulfate reducing bacteria including complete organic oxidizers (e.g. Desulfobacter) and incomplete organic oxidizers (e.g. Desulfovibrio) promoted sulfate reduction and organic/nutrient pollutants removal. Ammonia oxidizing bacteria (e.g. Nitrosomonas) and nitrite oxidizing bacteria (e.g. unidentified_Nitrospiraceae) were the potential nitrifiers for ammonia removal. Collaboration of anaerobic denitrifiers (e.g. Denitratisoma) and potential heterotrophic nitrifying and aerobic denitrifiers (HN-AD) achieved effective nitrate removal. This multi-unit treatment system with domestic sewage and silage fermentation broth as stimulation substrates provided an attractive option for AMD treatment.

Efficient reclaiming phosphate from aqueous solution using waste limestone modified sludge biochar: Mechanism and application as soil amendments.

A novel limestone-modified biochar derived from sewage sludge was prepared to reclaim phosphorus (P) from aqueous solution, and the potential application of P-laden biochar as soil amendments was also investigated. The limestone-modified biochar demonstrated excellent performance on phosphate recovery from aqueous solution in a wide range of pH (2.0-11.0), with maximum adsorption capacity of the biochar (Limestone/sludge mass ratio of 3:1) up to 231.28 mg P/g, which was 10.7 times that of the original sludge biochar. The adsorption was well described by the pseudo second-order model and Langmuir isotherm model. According to the adsorption thermodynamic parameters, the phosphate adsorption was spontaneous (ΔG0 < 0) and endothermic (ΔH0 > 0) so that increasing the temperature was beneficial to adsorption. Characterization analysis by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscope-energy dispersive spectrometer (SEM-EDS) proved that electrostatic attraction, surface complexation and brushite (CaHPO4.2H2O) precipitation were the dominant mechanism. The P-laden biochar exhibited an excellent ability to be reused as a new slow-release P fertilizer for soil. Pot experiment results showed that the treatment of P-laden LB 3:1 (P content of 22.8%) addition (1 wt%) significantly promoted Indian Lettuce germination (increasing by 14.4%), plant height (increasing by 18.6%), and dry biomass (53.0%) compared with the control, though it underperformed compared to commercial fertilizer.

Enhanced removal of phosphate from aqueous solution using Mg/Fe modified biochar derived from excess activated sludge: removal mechanism and environmental risk.

In this study, Mg-modified sludge biochar (MB) and Mg-Fe double oxides/sludge biochar composites (MFB) were synthesized for enhanced removal of phosphate from aqueous solution. The phosphate adsorption followed the Langmuir-Freundlich isotherm model, and the maximum capacity was 142.31 mg P/g and 35.41 mg P/g for MB and MFB, respectively. MB exhibited the higher adsorption capacity at pH 8-9 and performed well under the influences of coexisting anions and temperature (4-45 °C). Adsorption kinetics was well described by the pseudo-second-order kinetic model, indicating the chemical bonding between phosphate and adsorption sites. The adsorption capacity of phosphate decreased by < 15% after three successive recycles. Based on FTIR, XRD, and XPS analysis, the main mechanisms for phosphate removal by MB included electrostatic attraction, surface complexation, and precipitation. Hydroxides/oxides particles of Mg on the surface of MB with positive charge could adsorb HPO42- and PO43- to form surface complex and convert to MgHPO4 and Mg3(PO4)2. The released amounts of Fe, Cd, Cr, Pb, Cu, Zn, Sb, and As from MB and MFB were low and acceptable. However, the released amount of Mg was as high as 4.9 wt% for MB and 8.7 wt% for MFB at the pH corresponding maximum adsorption capacity, posing a risk of salt increase. The grass (Lolium perenne L.) germination and early growth with the addition of P-laden biochars as fertilizer are seriously inhibited due to the high alkalinity, particularly for MB. The environmental risk of P-laden biochars (with high alkalinity and salt content) as fertilizer should be emphasized in practical application.